GK091 ScienCell 人肝星狀細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Hepatic Stellate Cell Biology qPCR Array Kit

ScienCell 研究實驗室（ScienCell）是一家生物技術(shù)公司，其使命是實驗和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細(xì)胞，細(xì)胞培養(yǎng)基和試劑，培養(yǎng)基添加因子，細(xì)胞來源的RNA，cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量，實現(xiàn)細(xì)胞的能力，打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥，公司成立于1999年。

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GK091 ScienCell人肝星狀細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Hepatic Stellate Cell Biology qPCR Array Kit，產(chǎn)品簡介：

產(chǎn)品品牌： ScienCell

產(chǎn)品貨號：GK091

產(chǎn)品名稱：GK091 ScienCell人肝星狀細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Hepatic Stellate Cell Biology qPCR Array Kit

產(chǎn)品規(guī)格：One 96-well plate, one 20ul reaction per well

GK091 ScienCell人肝星狀細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Hepatic Stellate Cell Biology qPCR Array Kit， 產(chǎn)品說明：

ScienCell 的 GeneQuery™ 人肝星狀細(xì)胞生物學(xué) qPCR 芯片試劑盒 （GQH-HSB） 旨在促進 88 個關(guān)鍵基因的基因表達譜分析，這些基因涉及 （i） 人肝星狀細(xì)胞活化和增殖;（二）視黃醇代謝;（iii）細(xì)胞外基質(zhì)（ECM）合成和重塑;以及（iv）肝星狀細(xì)胞相關(guān)疾病。如何根據(jù)其功能對所包含的基因進行分組的簡要示例如下所示：  

肝星狀細(xì)胞標(biāo)志物：ACTA2，GFAP，DES，NGFR，COL2A1，VIM，LAMA1，NES

肝星狀細(xì)胞活化和增殖：ICAM1，MMP13，RELN，TIMP1，SYP，CDH1，RARA，PDGF，TGFB1，MMP2

肝臟發(fā)育和再生：STX2，PTN，SYNM，FOXF1，ICAM1，VCAM1，ITGB3，HGF，CXCL12，DES

視黃醇代謝： RBP1， RBP2， RBP4， RBP5， CRABP1， CRABP2， LRAT， CYP26

ECM合成和重塑：TGFB1，COL4A2，層粘連蛋白亞基，SPARC，VCAN，CTGF

細(xì)胞因子生產(chǎn)：EGF，IGF，PDGF，FGF，CSF1，CCL2，TNF，CXCL2，CXCL3，CXCL8

肝纖維化和癌癥發(fā)展：PKHD1，TMEM67，RPGRIP1L，SLC17A5，PKD1，CC2D2A，B9D1，B9D2

GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒，每個孔包含一個引物組，可以特異性識別并有效擴增靶基因的 cDNA。精心設(shè)計的引物確保：（i）qPCR分析中的優(yōu)良退火溫度為65°C（含2 mM Mg2+，不含DMSO）;（ii）除非另有說明，否則引物組可識別靶基因的所有已知轉(zhuǎn)錄本變體;（iii）只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

ScienCell's GeneQuery™ Human Hepatic Stellate Cell Biology qPCR Array Kit (GQH-HSB) is designed to facilitate gene expression profiling of 88 key genes involved in (i) human hepatic stellate cell activation and proliferation; (ii) retinol metabolism; (iii) extracellular matrix (ECM) synthesis and remodeling; and (iv) hepatic stellate cell related disorders. Brief examples of how included genes may be grouped according to their functions are shown below:    

·         Hepatic stellate cell markers: ACTA2, GFAP, DES, NGFR, COL2A1, VIM, LAMA1, NES

·         Hepatic stellate cell activation and proliferation: ICAM1, MMP13, RELN, TIMP1, SYP, CDH1, RARA, PDGFs, TGFB1, MMP2

·         Liver development and regeneration: STX2, PTN, SYNM, FOXF1, ICAM1, VCAM1, ITGB3, HGF, CXCL12, DES

·         Retinol metabolism: RBP1, RBP2, RBP4, RBP5, CRABP1, CRABP2, LRAT, CYP26

·         ECM synthesis and remodeling: TGFB1, COL4A2, Laminin subunits, SPARC, VCAN, CTGF

·         Cytokine production: EGF, IGFs, PDGFs, FGFs, CSF1, CCL2, TNF, CXCL2, CXCL3, CXCL8

·         Hepatic fibrosis and cancer development: PKHD1, TMEM67, RPGRIP1L, SLC17A5, PKD1, CC2D2A, B9D1, B9D2

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查詢™人肝星狀細(xì)胞生物學(xué)qPCR陣列板布局*（8 個粗體和斜體控件）

提示：基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

ScienCell基因表達分析產(chǎn)品訂購信息：

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