GK035 ScienCell 人體缺氧反應(yīng)qPCR檢測試劑盒,Human Hypoxia Response qPCR Array Kit

ScienCell 研究實(shí)驗室（ScienCell）是一家生物技術(shù)公司，其使命是實(shí)驗和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細(xì)胞，細(xì)胞培養(yǎng)基和試劑，培養(yǎng)基添加因子，細(xì)胞來源的RNA，cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量，實(shí)現(xiàn)細(xì)胞的能力，打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥，公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商，為客戶提供，正規(guī)進(jìn)口，貨期穩(wěn)定，折扣好的服務(wù)標(biāo)準(zhǔn)。

GK035 ScienCell人體缺氧反應(yīng)qPCR檢測試劑盒，Human Hypoxia Response qPCR Array Kit，產(chǎn)品簡介：

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號：GK035

產(chǎn)品名稱：GK035 ScienCell人體缺氧反應(yīng)qPCR檢測試劑盒，Human Hypoxia Response qPCR Array Kit

產(chǎn)品規(guī)格：One 96-well plate, one 20ul reaction per well

GK035 ScienCell人體缺氧反應(yīng)qPCR檢測試劑盒，Human Hypoxia Response qPCR Array Kit， 產(chǎn)品說明：

缺氧是指與正常有氧條件相比氧氣含量降低的任何環(huán)境。 當(dāng)細(xì)胞缺氧時，會誘導(dǎo)缺氧反應(yīng)，長時間缺氧通常會導(dǎo)致局部細(xì)胞環(huán)境的生理補(bǔ)償，例如缺氧區(qū)域的血管生成增加。 ScienCell 的 GeneQuery™ 人類缺氧反應(yīng) qPCR 陣列試劑盒 （GQH-HPX） 旨在促進(jìn)參與細(xì)胞對低氧條件反應(yīng)的 88 個關(guān)鍵基因的基因表達(dá)譜分析。 如何根據(jù)反應(yīng)類型對所包含的基因進(jìn)行分組的簡要示例如下所示：

細(xì)胞代謝： ENO1， HK1， PFKFB3， PGK1， SLC2A1

炎癥： CXCL8， IL1A， CCL2， IL6， THBD

血管松弛：AHSP，PTGIS

細(xì)胞死亡或增殖：BNIP3L、NOS3、PIM1、EPO

血管生成： KDR， PDGFB， PLAU， TEK， FLT1

GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒，每個孔包含一個引物組，可識別并有效擴(kuò)增特定靶基因的 cDNA。精心設(shè)計的引物確保：（i）qPCR分析中的優(yōu)良退火溫度為65°C（含2 mM Mg2+且不含DMSO）;（ii）除非另有說明，否則引物集可識別靶基因的所有已知轉(zhuǎn)錄本變體;（iii）只有一個基因被擴(kuò)增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗證。

Hypoxia refers to any environment with reduced oxygen content compared to normal aerobic conditions.  The hypoxic response is induced when cells are oxygen deprived and prolonged deprivation often results in physiological compensation by the local cellular environment, such as increased angiogenesis to the hypoxic region.  ScienCell's GeneQuery™ Human Hypoxia Response qPCR Array Kit (GQH-HPX) is designed to facilitate gene expression profiling of 88 key genes involved in the cellular response to low-oxygen conditions.  Brief examples of how included genes may be grouped according to response type are shown below:

·         Cellular metabolism: ENO1, HK1, PFKFB3, PGK1, SLC2A1

·         Inflammation: CXCL8, IL1A, CCL2, IL6, THBD

·         Vasorelaxation: AHSP, PTGIS

·         Cell death or proliferation: BNIP3L, NOS3, PIM1, EPO

·         Angiogenesis: KDR, PDGFB, PLAU, TEK, FLT1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人內(nèi)皮細(xì)胞生物學(xué) qPCR 陣列板布局*（8 個粗體和斜體對照）

提示：基因選擇可能會根據(jù)新的研究和開發(fā)進(jìn)行更新

ScienCell基因表達(dá)分析產(chǎn)品訂購信息：

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