ScienCell GK025 人類Notch信號通路qPCR檢測試劑盒,uman Notch Signaling Pathway qPCR Array Kit

ScienCell 研究實(shí)驗(yàn)室（ScienCell）是一家生物技術(shù)公司，其使命是實(shí)驗(yàn)和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細(xì)胞，細(xì)胞培養(yǎng)基和試劑，培養(yǎng)基添加因子，細(xì)胞來源的RNA，cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量，實(shí)現(xiàn)細(xì)胞的能力，打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥，公司成立于1999年。

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GK025 ScienCell人類Notch信號通路qPCR檢測試劑盒，uman Notch Signaling Pathway qPCR Array Kit，產(chǎn)品簡介：

產(chǎn)品品牌:  ScienCell

產(chǎn)品貨號：GK025

產(chǎn)品名稱：GK025 ScienCell人類Notch信號通路qPCR檢測試劑盒，uman Notch Signaling Pathway qPCR Array Kit

產(chǎn)品規(guī)格：One 96-well plate, one 20ul reaction per well

GK025 ScienCell人類Notch信號通路qPCR檢測試劑盒，uman Notch Signaling Pathway qPCR Array Kit， 產(chǎn)品說明：

ScienCell 的 GeneQuery™ Human Notch 信號通路 qPCR 芯片試劑盒 （GQH-NCH） 旨在促進(jìn)參與人類 Notch 分子通路的 88 個關(guān)鍵基因的基因表達(dá)譜分析，重點(diǎn)是 Notch1 信號轉(zhuǎn)導(dǎo)。 如何根據(jù)其作用對所包含的基因進(jìn)行分組的簡要示例如下所示：

規(guī)范配體：JAG1、JAG2、DLL1、DLL3DLL4

非規(guī)范配體：WNT1，DNER，CNTN1，NOV，SHH

受體：缺口1，缺口2，缺口3，缺口4

轉(zhuǎn)錄抑制因子：HEY1、HEY2、ESR1、HES1、HES7

蛋白水解：ADAM17、PSEN1、FURIN、POFUT1、PSENEN

GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒，每個孔包含一個引物組，可識別并有效擴(kuò)增特定靶基因的 cDNA。精心設(shè)計的引物確保：（i）qPCR分析中的優(yōu)良退火溫度為65°C（含2 mM Mg2+且不含DMSO）;（ii）除非另有說明，否則引物集可識別靶基因的所有已知轉(zhuǎn)錄本變體;（iii）只有一個基因被擴(kuò)增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗(yàn)證。

ScienCell's GeneQuery™ Human Notch Signaling Pathway qPCR array kit (GQH-NCH) is designed to facilitate gene expression profiling of 88 key genes involved in the human Notch molecular pathway with an emphasis on Notch1 signal transduction.  Brief examples of how included genes may be grouped according to their roles are shown below:

·         Canonical ligands: JAG1, JAG2, DLL1, DLL3DLL4

·         Noncanonical ligands: WNT1, DNER, CNTN1, NOV, SHH

·         Receptors: NOTCH1, NOTCH2, NOTCH3, NOTCH4

·         Transcriptional repressors: HEY1, HEY2, ESR1, HES1, HES7

·         Proteolysis: ADAM17, PSEN1, FURIN, POFUT1, PSENEN

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人類缺口信號通路 qPCR 陣列板布局*（8 個粗體和斜體對照）

提示：基因選擇可能會根據(jù)新的研究和開發(fā)進(jìn)行更新

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